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After reaching 70% confluence, fibroblasts were cultured for 24 h in 1% FBS DMEM and then incubated with either TGF1 10ng/ml (Peprotech, Dollard des Ormeaux, Quebec, Canada), GHK 10 nM (Sigma, Markham, Ontario, Canada) or control media (1% FBS DMEM, penicillin, streptavidin) for a further 48 h

Unlike the traditional one drug-one target-one disease paradigm, network-based drug discovery reveals disease mechanisms from a holistic perspective, fully considering the complexity of biological systems [10]

Tips for Equine Injections Consult Your Veterinarian: Before administering any injections, consult your veterinarian to ensure they are appropriate for your horse's specific needs